Tutor A. Spermatozoa were first described by Leeuwenhoek in the 17th century but it was not until that the sperm count was found to be associated with fertility potential Since that time a variety of sperm tests and semen parameters have been developed with the hope of clarifying whether or not a man could impregnate his partner. MacLeod , MacLeod and Gold , Eliasson and Hellinga , have led the scientific basis of conventional analysis of spermatozoa and the techniques recommended by them are still considered the reference for more advanced methods 6.
Under natural mating, with a ratio of hen:cocks of fertility rates are still low. Artificial insemination AI technology can be adopted and is the most effective means for economic poultry production and genetic improvement. The effect of different dilution rates of freshly ejaculated white leghorn chicken WLH semen was investigated using CARI poultry semen diluent. Healthy adult males and healthy adult hens from the same hatch were taken randomly and maintained in individual cages under uniform husbandry conditions. The hens were divided equally and randomly into 9 groups. Good quality pooled fresh semen samples were collected by massage method and diluted with CARI poultry semen diluent at different dilution rates.
Hemacytometers were developed for counting blood cells, but can also be used to count spermatozoa. A hemacytometer has two chambers and each chamber has a microscopic grid etched on the glass surface. The chambers are overlaid with a glass coverslip that rests on pillars exactly 0. Thus, the volume of fluid above each square of the grid is known with precision. The semen must be killed to prevent movement and diluted before loading into the hemacytometer.
Diluting a Specimen for Counting on a Hemocytometer. Semen must be diluted prior to counting sperm if a hemocytometer is used. In additon to preventing overlapping of sperm cells, the diluting fluid immobilizes the sperm in the chamber to further facilitate counting. Following liquefaction at about 30 minutes , mix the sample manually by swirling the container several times. Thorough mixing is essential for accurate counting.